LncRNA XIST acts as a MicroRNA-520 sponge to regulate the Cisplatin resistance in NSCLC cells by mediating BAX through CeRNA network

Background: In recent years, LncRNA acts as a member of competing endogenous RNA (ceRNA), playing an important role in drug resistance lung cancer. The aim this study was to identify potential biomarkers about cisplatin resistant cancer cells using comprehensive ceRNA network. Methods: GSE6410 (GPL-201) analyzed gene expression changes A549 NSCLC cells. GSE43249 (GPL-14613) included noncoding profiling derived from the GEO2R, online analysis tool, differentially expressed mRNAs and miRNAs (DEmRNAs DEmiRNAs). To explore functional enrichment implication mRNAs, we used GO (Gene ontology) KEGG (Kyoto Encyclopedia Genes Genomes) pathway analysis. Through miRDB, Targetscan, Starbase miRWalk, found targeted miRNAs. Kaplan-Meier curve method show clinical survival RNAs (P<0.05). database predicted lncRNAs mediated Eventually, novel network lncRNAs, miRNAs, mRNA constructed by cytoscape3.7.2. Results: 118 were basis DAVID picked out BAX, apoptosis regulator. Venn diagram demonstrated 8 commonly regulating BAX. lncRNA XIST Finally, may be useful biomarker further, explored BAX effect tumor-infiltrating immune Conclusions: competitively bound miRNA 520 regulation participating p53 signaling pathway.